Affiliation:
1. Centre de Recherche en Virologie, Institut Armand-Frappier, Laval, Quebec, Canada.
Abstract
Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days, colonies were transferred to nitrocellulose membranes by blotting. Simultaneous detection and identification of L. pneumophila were done by treating the membrane with the monoclonal antibody and a peroxidase conjugate to mouse immunoglobulins. A diffuse cross-reaction was observed with Pseudomonas fluorescens colonies, but this was a low-level reaction that could easily be differentiated from the strong specific reactions to L. pneumophila.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference8 articles.
1. American Public Health Association. 1985. Standard methods for the examination of water and wastewater 16th ed. p. 934-935. American Public Health Association Washington D.C.
2. Common epitope on the lipopolysaccharide of Legionella pneumophila recognized by a monoclonal antibody;Barthe C.;J. Clin. Microbiol.,1988
3. Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium;Bopp C. A.;J. Clin. Microbiol.,1981
4. Enhancement of recovery of Legionella pneumnophila from contaminated respiratory tract specimens by heat;Edelstein P. H.;J. Clin. Microbiol.,1982
5. Fliermans C. B. 1985. Ecological niche of Legionella pneulmophila p. 73-116. In S. M. Katz (ed.) Legionellosis vol. 2. CRC Press Inc. Boca Raton Fla.
Cited by
8 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献