Affiliation:
1. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama 227, Japan
Abstract
The ultrasonic determination of microbial population was carried out by using a new apparatus composed of two piezoelectric membranes. When the apparatus was immersed in a medium containing cells and an arbitrary voltage was charged on a piezoelectric membrane, an output voltage was obtained from the other membrane. The output voltage increased with increasing cell numbers. The linear relationships between the output voltage and microbial populations of
Saccharomyces cerevisiae, Bacillus subtilis
, and
Klebsiella
sp. were observed, and the output voltage was reproducible with a relative error of 6%. Furthermore, the cell population of
S. cerevisiae
in a fermentor could be continuously determined with this apparatus.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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