Affiliation:
1. Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854
Abstract
ABSTRACT
Protein S is a major spore coat protein of
Myxococcus xanthus
, consisting of two homologous domains, the N-terminal domain (NTD) and the C-terminal domain, both of which contain a Ca
2+
-binding site. Protein S tightly binds to myxospores in a Ca
2+
-dependent manner. Here, we constructed a novel expression vector, pCold-PST, encoding two tandem repeat NTDs (PrS
2
). By using this vector, a number of human proteins that were expressed at low levels or in insoluble forms by a pET vector were expressed not only at high levels but also in soluble forms. We also demonstrated that an
Escherichia coli
protein tagged with PrS
2
fully retained its function, indicating that it is folded independently from the tag. This technology not only allows simple, one-step protein purification using myxospores, but can also be used for the identification of proteins interacting with a protein of interest and will prove immensely useful for structural studies of proteins which are difficult to produce or are insoluble.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
24 articles.
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