Comparative Evaluation of Semiautomated COBAS AMPLICOR Hepatitis B Virus (HBV) MONITOR Test and Manual Microwell Plate-Based AMPLICOR HBV MONITOR Test-Reference-Cited by-同舟云学术

Comparative Evaluation of Semiautomated COBAS AMPLICOR Hepatitis B Virus (HBV) MONITOR Test and Manual Microwell Plate-Based AMPLICOR HBV MONITOR Test

Published:2001-02 Issue:2 Volume:39 Page:758-761
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Marin Irena J.¹,Poljak Mario¹,Seme Katja¹,Meglic̆-Volkar Jelka²,Matic̆ic̆ Mojca²,Les̆nic̆ar Gorazd³,Brinovec Vladimir²

Affiliation:

1. Institute of Microbiology and Immunology, Medical Faculty,1 and

2. Department of Infection Diseases, Clinical Center,2 Ljubljana, and

3. Department of Infection Diseases, General Hospital, Celje,3 Slovenia

Abstract

ABSTRACT Comparative evaluation of the semiautomated COBAS AMPLICOR hepatitis B virus (HBV) MONITOR Test (COBAS-HBV) and manual AMPLICOR HBV MONITOR Test (AMPLICOR-HBV) on 208 serum samples revealed no significant difference in the sensitivities of the two assays. Twenty samples tested HBV DNA negative and 183 samples tested HBV DNA positive by both assays. Three samples tested positive by COBAS-HBV only and two samples tested positive by AMPLICOR-HBV only. HBV DNA concentrations determined by the two assays were significantly related ( n = 183, r = 0.97, P < 0.0001), which indicates that COBAS-HBV could replace AMPLICOR-HBV. The major inconvenience of COBAS-HBV is the required performance of appropriate predilutions of high-titer samples in order to extend the narrow dynamic range of the assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.2.758-761.2001

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