Oxford Nanopore Sequencing Reveals an Exotic Broad bean mottle virus Genome within Australian Grains Post-Entry Quarantine

Author:

Maina S.12ORCID,Norton S. L.1,McQueen V. L.1,King S.1,Rodoni B.34

Affiliation:

1. Australian Grains Genebank, Agriculture Victoria Research, Horsham, Victoria, Australia

2. Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, New South Wales, Australia

3. Microbial Pests & Diseases, Agriculture Victoria Research, AgriBio, Bundoora, Victoria, Australia

4. School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, Australia

Abstract

A Broad bean mottle virus (BBMV) isolate (S52) obtained from an infected Vicia faba leaf sample from Syria was sequenced using Oxford Nanopore long-read sequencing at the Australian border. The genome had 95.6%, 98.2%, and 93.4% nucleotide sequence identity to BBMV strains RNA1 (Bawden), RNA2 (Mo), and RNA3 (Bawden).

Funder

Grains Research and Development Corporation

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Reference15 articles.

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4. Broad bean mottle virus in Morocco; curculionid vectors, and natural occurrence in food legumes other than faba bean (Vicia faba)

5. Transcriptome Sequencing Reveals the Genome Sequence of Pea Early Browning Virus from a 29-Year-Old Faba Bean Sample

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