Oxford Nanopore Sequencing Reveals an Exotic <i>Broad bean mottle virus</i> Genome within Australian Grains Post-Entry Quarantine-Reference-Cited by-同舟云学术

Oxford Nanopore Sequencing Reveals an Exotic Broad bean mottle virus Genome within Australian Grains Post-Entry Quarantine

Published:2022-07-21 Issue:7 Volume:11 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Maina S.¹²^ORCID,Norton S. L.¹,McQueen V. L.¹,King S.¹,Rodoni B.³⁴

Affiliation:

1. Australian Grains Genebank, Agriculture Victoria Research, Horsham, Victoria, Australia

2. Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, New South Wales, Australia

3. Microbial Pests & Diseases, Agriculture Victoria Research, AgriBio, Bundoora, Victoria, Australia

4. School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, Australia

Abstract

A Broad bean mottle virus (BBMV) isolate (S52) obtained from an infected Vicia faba leaf sample from Syria was sequenced using Oxford Nanopore long-read sequencing at the Australian border. The genome had 95.6%, 98.2%, and 93.4% nucleotide sequence identity to BBMV strains RNA1 (Bawden), RNA2 (Mo), and RNA3 (Bawden).

Funder

Grains Research and Development Corporation

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.00211-22

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