Identification of Bacterial Taxa Present in a Concrete Pennsylvania Bridge-Reference-Cited by-同舟云学术

Identification of Bacterial Taxa Present in a Concrete Pennsylvania Bridge

Published:2023-06-20 Issue:6 Volume:12 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Tadley Patricia¹,Bennett Alexa²,Hansen Kirstin¹,Hanson Thomas²^ORCID,Colosi Joseph¹,Goudsouzian Lara K.¹^ORCID

Affiliation:

1. Department of Biology, DeSales University, Center Valley, Pennsylvania, USA

2. School of Marine Science and Policy, University of Delaware, Newark, Delaware, USA

Abstract

Concrete contains low microbial biomass, but some bacteria can grow in this highly alkaline environment. We used silica-based DNA extraction and 16S rRNA sequence analysis to identify the bacteria in a corroded concrete bridge sample from Bethlehem, Pennsylvania. Staphylococcus , Streptococcus , Corynebacterium , Leifsonia , Vicinamibacterales , and Actinophytocola were the most abundant genera.

Funder

National Science Foundation

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.00211-23

Reference14 articles.

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4. Bacterial Communities in Concrete Reflect Its Composite Nature and Change with Weathering

5. Protocols.io. 2021. DNA extraction from concrete. https://www.protocols.io/view/dna-extraction-from-concrete-5jyl853e8l2w/v1?version_warning=no.