Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system-Reference-Cited by-同舟云学术

Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system

Published:1977-04 Issue:1 Volume:22 Page:97-101
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Clinkscales C W,Bratt M A,Morrison T G

Abstract

We have isolated 18S RNA from cytoplasmic extracts of Newcastle disease virus-infected Chinese hamster ovary cells and tested its ability to direct protein synthesis in extracts derived from wheat germ. The products of the cell-free reaction directed by this RNA contain polypeptides that comigrate with NP, M,F, and 47K roteins from virions. In addition, the products contain a polypeptide (67K) that migrates on polyacrylamide gels slightly faster than the HN protein from virions. Tryptic peptide analysis of the cell-free products and proteins from virions confirms their identity.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.22.1.97-101.1977

Reference24 articles.

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