Rapid Technique for the Enumeration of Clostridium perfringens-Reference-Cited by-同舟云学术

Rapid Technique for the Enumeration of Clostridium perfringens

Published:1965-07 Issue:4 Volume:13 Page:559-563
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Marshall Robert S.¹,Steenbergen J. Frank¹,McClung L. S.¹

Affiliation:

1. Department of Bacteriology, Indiana University, Bloomington, Indiana

Abstract

A new medium, Tryptone-sulfite-neomycin (TSN) agar, and an incubation procedure for the enumeration of Clostridium perfringens are described. Tolerance to neomycin, optimal growth at 46 C, and sulfite-reducing properties of C. perfringens were used as a basis for development of the medium. Comparisons were made between sulfite-polymyxin-sulfadiazine (SPS) agar and TSN agar at 37 and 46 C with C. perfringens and other organisms. These studies indicate the quantitative and selective superiority of TSN agar, incubated at 46 C, over SPS agar.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.13.4.559-563.1965

Reference20 articles.

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3. ARBUCKLE R. E. 1960. The influence of temperature on the growth of Clostridium perfringens. M.A. Thesis Indiana Univ. Bloomington.

4. Contribution a l'etude des milieux au sulfite de sodium pour l'isolement des;BEERENS H., M.;Clostridium. Ann. Inst. Pasteur Lille,1961

5. The growth requirements of Clostridium perfringens (welchii) BP6K;BOYD M. J.;J. Biol. Chem.,1948

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