External Quality Assessment Program for Chlamydia trachomatis Diagnostic Testing by Nucleic Acid Amplification Assays-Reference-Cited by-同舟云学术

External Quality Assessment Program for Chlamydia trachomatis Diagnostic Testing by Nucleic Acid Amplification Assays

Published:2002-08 Issue:8 Volume:40 Page:2893-2896
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Land Sally¹,Tabrizi Sepehr²,Gust Anthony¹,Johnson Elizabeth¹,Garland Susan²,Dax Elizabeth M.¹

Affiliation:

1. National Serology Reference Laboratory, Australia, St Vincent's Institute of Medical Research, Fitzroy, VIC 3065

2. Department of Microbiology and Infectious Diseases, Women's and Children's Health Care Network, Royal Women's Hospital, Carlton, VIC 3053, Australia

Abstract

ABSTRACT We report the results from 57 Australian diagnostic laboratories testing two external quality assessment panels using either the Roche Amplicor Chlamydia trachomatis test (R-PCR) or the Abbott LCx Chlamydia trachomatis assay (A-ligase chain reaction [LCR]). Panel samples were either normal urine spiked with Chlamydia trachomatis antigen or clinical urine specimens. There was no significant difference between laboratories or between assays in detection of C. trachomatis- positive clinical samples. Only at the lower limit of detection of the assays did the R-PCR demonstrate increased sensitivity over the A-LCR in the detection of C. trachomatis antigen. However, it was found that single-sample testing could lead to decreased test sensitivity. Detection of the presence of inhibitors of nucleic acid amplification differed between laboratories.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.8.2893-2896.2002

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