High incidence of transposon Tn3 insertions into a replication control gene of the chimeric R/Ent plasmid pCG86 of Escherichia coli

Author:

Picken R N,Mazaitis A J,Maas W K

Abstract

Insertion of transposon Tn3 into the chimeric R/Ent plasmid pCG86 occurred preferentially into a replication control gene, generating mutants with increased plasmid copy number. In two mutants, the nucleotide sequence of the region of the gene containing the Tn3 insertion was determined.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference17 articles.

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4. DNA sequence analysis of the transposon Tn 3: three genes and three sites involved in transposition of Tn 3;Heffron F.;Cell,1979

5. Hirst T. R. L. L. Randall and S. J. S. Hardy. 1984. Cellular NOTES 433

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