Novel Amiloride Derivatives That Inhibit Bacterial Motility across Multiple Strains and Stator Types

Author:

Islam M. I.1,Bae J. H.1,Ishida T.2,Ridone P.1,Lin J.1,Kelso M. J.345,Sowa Y.26ORCID,Buckley B. J.345ORCID,Baker M. A. B.17ORCID

Affiliation:

1. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia

2. Department of Frontier Bioscience, Hosei University, Tokyo, Japan

3. Molecular Horizons, University of Wollongong, Wollongong, New South Wales, Australia

4. School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, New South Wales, Australia

5. Illawarra Health and Medical Research Institute, Wollongong, New South Wales, Australia

6. Research Center for Micro-Nano Technology, Hosei University, Tokyo, Japan

7. CSIRO Synthetic Biology Future Science Platform, Brisbane, Queensland, Australia

Abstract

Here, we characterized two novel amiloride derivatives in the search for antimicrobial compounds that target bacterial motility. Our two compounds were shown to inhibit flagellar motility at 10 μM across multiple strains: from nonpathogenic Escherichia coli with flagellar rotation driven by proton or chimeric sodium-powered stators, to proton-powered pathogenic E. coli (enterohemorrhagic E. coli or uropathogenic E. coli [EHEC or UPEC, respectively]), and finally, sodium-powered Vibrio alginolyticus .

Funder

Australian Research Council

Australian National Health and Medical Research Council

MEXT | Japan Society for the Promotion of Science

Takeda Science Foundation

Commonwealth Scientific and Industrial Research Organisation

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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