Polyacrylamide Slab Gel Electrophoresis of Soluble Proteins for Studies of Bacterial Floras-Reference-Cited by-同舟云学术

Polyacrylamide Slab Gel Electrophoresis of Soluble Proteins for Studies of Bacterial Floras

Published:1980-04 Issue:4 Volume:39 Page:900-907
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Moore W. E. C.¹,Hash D. E.¹,Holdeman Lillian V.¹,Cato Elizabeth P.¹

Affiliation:

1. Anaerobe Laboratory, College of Agriculture and Life Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

Abstract

A polyacrylamide slab gel electrophoresis procedure was used to compare cellular proteins from bacterial isolates of gingival crevice floras. Isolates with identical protein patterns consistently were shown to be members of the same species. When used to screen isolates, the procedure reduced total analytical time and expense without sacrificing accuracy, and it provided additional verification of the identity of strains characterized by conventional phenotypic tests.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.39.4.900-907.1980

Reference19 articles.

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3. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs;Ames G.;J. Biol. Chem.,1974

4. Disc electrophoresis-II. Method and application to human serum proteins;Davis B. J.;Ann. New York Acad. Sci.,1964

5. Evaluation of the taxonomic relationship of Micrococcus cryophilus, Branhamella catarrhalis, and Neisseriae by comparative polyacrylamide gel electrophoresis of soluble proteins;Fox R. H.;Int. J. Syst. Bacteriol.,1974

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