Efficiency of homologous intermolecular recombination at different locations on the Bacillus subtilis chromosome-Reference-Cited by-同舟云学术

Efficiency of homologous intermolecular recombination at different locations on the Bacillus subtilis chromosome

Published:1992-09 Issue:17 Volume:174 Page:5593-5596
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Biswas I¹,Vagner V¹,Ehrlich S D¹

Affiliation:

1. Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

Abstract

The efficiencies of intermolecular recombination at 12 different locations on the Bacillus subtilis chromosome were determined by transforming competent cells with a nonreplicative plasmid. The efficiencies varied by only about threefold but were significantly different (P less than 0.05 by a chi-square test) for approximately 20% of the locations. The recA gene product is required for recombination, and the addA gene product appears to affect the variation in a site-specific way.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.174.17.5593-5596.1992

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