Sequence and characterization of pvuIIR, the PvuII endonuclease gene, and of pvuIIC, its regulatory gene

Author:

Tao T1,Blumenthal R M1

Affiliation:

1. Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.

Abstract

An open reading frame partially overlaps pvuIIR, and genetic evidence implies that this open reading frame, named pvuIIC, specifies a positive regulator of pvuIIR (T. Tao, J. C. Bourne, and R. M. Blumenthal, J. Bacteriol. 173:1367-1375, 1991). Inducible constructs of pvuIIC produced a protein of the expected size. The site of C.PvuII action appears to lie within pvuIIC itself; thus, pvuIIC may be a self-contained regulatory cassette.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference24 articles.

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3. Benner J. S. L. Dorner L. Greenough D. Heiter L. Hornstra and W. Jack. (New England BioLabs Inc.). 1990. Unpublished data.

4. Cloning of a restriction-modification system from Proteus vulgaris and its use in analyzing a methylase-sensitive phenotype in Escherichia coli;Blumenthal R. M.;J. Bacteriol.,1985

5. Characterization of the genes coding for the EcoRV restriction and modification system of Escherichia coli;Bougueleret L.;Nucleic Acids Res.,1984

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