Rapid and Simple Quantification of Bacterial Cells by Using a Microfluidic Device-Reference-Cited by-同舟云学术

Rapid and Simple Quantification of Bacterial Cells by Using a Microfluidic Device

Published:2005-02 Issue:2 Volume:71 Page:1117-1121
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Sakamoto Chieko¹,Yamaguchi Nobuyasu¹,Nasu Masao

Affiliation:

1. Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan

Abstract

ABSTRACT This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.2.1117-1121.2005

Reference24 articles.

1. Auroux, P. A., D. Iossifidis, D. R. Reyes, and A. Manz. 2002. Micro total analysis systems. 2. Analytical standard operations and applications. Anal. Chem.74:2637-2652.

2. Cabrera, C. R., and P. Yager. 2001. Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques. Electrophoresis22:355-362.

3. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

4. Dittrich, P. S., and P. Schwille. 2003. An integrated microfluidic system for reaction, high-sensitivity detection, and sorting of fluorescent cells and particles. Anal. Chem.75:5767-5774.

5. Effenhauser, C. S., A. Paulus, A. Manz, and H. M. Widmer. 1994. High-speed separation of antisense oligonucleotides on a micromachined capillary electrophoresis device. Anal. Chem.66:2949-2953.

Cited by 55 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Viable Escherichia coli enumeration on a polydimethylsiloxane (PDMS) chip with vertical channel-well configuration;Microchimica Acta;2024-04-04

2. Simple Staining of Cells on a Chip;Biosensors;2022-11-13

3. Fabrication of a new all-in-one microfluidic dielectrophoresis integrated chip and living cell separation;iScience;2022-02

4. Environmental Monitoring in a Pharmaceutical Manufacturing Facility Using a Culture Independent Approach;YAKUGAKU ZASSHI;2022-01-01

5. A critical review: Recent advances in “digital” biomolecule detection with single copy sensitivity;Biosensors and Bioelectronics;2021-04