Prototrophic Thermophilic Bacillus: Isolation, Properties, and Kinetics of Growth

Author:

Epstein Israel1,Grossowicz Nathan1

Affiliation:

1. Department of Bacteriology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

Abstract

A thermophilic bacillus (minimal growth temperature 41 C, optimal 55 to 58 C, and maximal 65 C) was isolated from a manure pile. It is very similar to Bacillus stearothermophilus , but it differs in its inability to hydrolyze starch. The thermophilic isolate is a prototroph which grows in a minimal medium consisting of glucose, ammonium salt, phosphate buffer, and inorganic salts. At all temperatures studied (low to high), the same minimal nutritional requirements prevailed. The Arrhenius constant for growth was found to be 15,000 and 13,500 cal/mole in the minimal and rich media, respectively.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference18 articles.

1. The thermophilic aerobic sporeforming bacteria;Allen M. B.;Bacteriol. Rev.,1953

2. Growth requirements of 94 strains of thermophilic bacilli;Baker H.;Can. J. Microbiol.,1960

3. An assay for pantothenic acid in biological fluids;Baker H.;Clin. Chem.,1960

4. Nutritional factors in thermophily: a comparative study of Bacilli and;Baker H.;Euglena. Ann. N. Y. Acad. Sci.,1955

5. Growth requirements of some thermophilic and mesophilic bacilli;Baker H.;J. Gen. Microbiol.,1953

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