Spontaneous and induced rho mutants of Saccharomyces cerevisiae: patterns of loss of mitochondrial genetic markers

Author:

Heude M,Fukuhara H,Moustacchi E

Abstract

The deletion which leads to spontaneous rho mutants occurs preferentially at a unique region covering genes oxi3, pho1/OII, and mit175. The frequency of loss of genetic markers in this region was significantly higher than in other regions as determined with a 15- marker system. When various mutagenic treatments were applied, this specific pattern of deletion was also observed, but it was dramatically amplified. This suggests that the basic mechanism of rho production is the same in yeast mitochondrial genomes in both spontaneous and induced mutants.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference28 articles.

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2. Modified recombination and transmission of mitochondrial genetic markers in rho minus mutants of Saccharomyces cerevisiae;Bolotin-Fukuhara M.;Proc. Natl. Acad. Sci. U.S.A.,1976

3. Temperature-sensitive respiratory-deficient mitochondrial mutations: isolation and genetic mapping;Bolotin-Fukuhara M.;Mol. Gen. Genet.,1977

4. Borst P. 1974. Structure ofpetite mt DNA; prospects for mitochondrial gene isolation mapping and sequencing p. 147-156. In A. M. Kroon and C. Saccone (ed.) The biogenesis of mitochondria. Academic Press Inc. New York.

5. Petite deletion map of the mitochondrial oxi 3 region in Saccharomyces cerevisiae;Carignani G.;Mol. Gen. Genet.,1979

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