Application ofpheBas a Reporter Gene for Geobacillus spp., Enabling Qualitative Colony Screening and Quantitative Analysis of Promoter Strength

Abstract

ABSTRACTThepheBgene fromGeobacillus stearothermophilusDSM6285 has been exploited as a reporter gene forGeobacillusspp. The gene product, catechol 2,3-dioxygenase (C23O), catalyzes the formation of 2-hydroxymuconic semialdehyde, which can be readily assayed. The reporter was used to examine expression from theldhpromoter associated with fermentative metabolism.