Genetic Mapping of a Defective Bacteriophage on the Chromosome of Bacillus subtilis 168

Author:

Garro Anthony J.1,Leffert Hyam1,Marmur Julius1

Affiliation:

1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461

Abstract

A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2 , which were linked by transformation and PBS1-mediated transduction to metC . The link-age relationship between xtl and adjacent auxotrophic markers was determined by three-factor PBS1 transduction, the suggested order of markers being argO 1 metA metC xtl.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference38 articles.

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3. The isolation and morphology of some new bacteriophages specific for Bacillus and Acetobacter species;Bradley D. E.;J. Gen. Microbiol.,1965

4. The structure of phage-like objects associated with non-induced bacteriocinogenic bacteria;Bradley D. E.;J. Gen. Microbiol.,1966

5. Campbell A. M. 1969. Episomes. Harper and Row New York.

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