Affiliation:
1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461
Abstract
A genetic marker responsible for the killing activity of PBSX, a defective phage carried by
Bacillus subtilis
168, has been located on the bacterial chromosome. Two mutant strains of
B. subtilis
168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated
xtl-1
and
xtl-2
, which were linked by transformation and PBS1-mediated transduction to
metC
. The link-age relationship between
xtl
and adjacent auxotrophic markers was determined by three-factor PBS1 transduction, the suggested order of markers being
argO 1 metA metC xtl.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
31 articles.
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