Comparison of various techniques for determining viability of Paracoccidioides brasiliensis yeast-form cells

Author:

Restrepo A,Cano L E,de Bedout C,Brummer E,Stevens D A

Abstract

The viability of Paracoccidioides brasiliensis yeast-form cells was determined by colony-forming units, direct fluorescent staining, and production of germ tubes in slide culture. The first procedure was unreliable and time consuming; the latter two showed better correlation with hemacytometer total cell counts and required significantly less time.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference9 articles.

1. Determination of the growth curves of the mycelial and yeast forms of P. brasiliensis;Arango M.;Mycopathologia,1976

2. A new fluorescent viability test for fungi cells;Calich V. L. G.;Mycopathologia,1978

3. Emmons C. W. C. H. Blnford and J. P. Utz. 1979. Medical mycology 3rd ed. Lea & Febiger Philadelphia. Pa.

4. Studies on plating efficiency and estimation of viability of suspensions of P. brasiliensis yeast-cells;Goihmann-Yahr M.;Mycopathologia,1980

5. Growth curves and nucleic acid content of mycelial and yeast-like forms of P. brasiliensis;Ramirez-Martinez J. R.;Mycopathologia,1970

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