Author:
Balachandran N,Frame B,Chernesky M,Kraiselburd E,Kouri Y,Garcia D,Lavery C,Rawls W E
Abstract
Monoclonal antibodies which reacted with type-specific antigens of herpes simplex virus type 2 or with antigens shared by herpes simplex virus types 1 and 2 were used in an indirect immunofluorescence assay to type virus isolates and to detect viral antigens in cells obtained from herpetic lesions. Complete concordance was obtained for 42 isolates typed by endonuclease restriction analysis of viral DNA and by indirect immunofluorescence with monoclonal antibodies. Examination of a limited number of ulcerative lesions revealed that indirect immunofluorescence and virus isolation were comparable in detecting herpes simplex virus. The results indicate that monoclonal antibodies can be used to accurately identify and type isolates of herpes simplex virus.
Publisher
American Society for Microbiology
Reference34 articles.
1. Persistence of virus shedding in asymptomatic women after recovery from herpes genitalis;Adam E.;Obstet. Gynecol.,1979
2. Back A. F. and N. J. Schmidt. 1974. Typing herpesvirus 208 NOTES
3. Monoclonal antibodies in two glycoproteins of herpes simplex virus type 2;Balachandran N.;J. Virol.,1981
4. Rapid typing of herpes simplex virus strains using the indirect immunoperoxidase method;Benjamin D. R.;Appl. Microbiol.,1974
5. Rapid diagnosis of herpes simplex virus infection with fluorescent antibody;Biegeleison J. Z.;Science,1959
Cited by
66 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献