Detection of mecA - and mecC -Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay-Reference-Cited by-同舟云学术

Detection of mecA - and mecC -Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay

Published:2016-01 Issue:1 Volume:54 Page:180-184
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Becker Karsten¹,Denis Olivier²,Roisin Sandrine²,Mellmann Alexander³^ORCID,Idelevich Evgeny A.¹,Knaack Dennis¹,van Alen Sarah¹,Kriegeskorte André¹,Köck Robin¹,Schaumburg Frieder¹,Peters Georg¹,Ballhausen Britta¹

Affiliation:

1. Institute of Medical Microbiology, University Hospital Münster, Münster, Germany

2. Staphylococcus National Reference Centre, Erasme Hospital, Université Libre de Bruxelles, Brussels, Belgium

3. Institute of Hygiene, University Hospital Münster, Münster, Germany

Abstract

ABSTRACT An advanced methicillin-resistant Staphylococcus aureus (MRSA) detection PCR approach targeting SCC mec-orfX along with mecA and mecC was evaluated for S. aureus and coagulase-negative staphylococci. The possession of mecA and/or mecC was correctly confirmed in all cases. All methicillin-susceptible S. aureus strains ( n = 98; including staphylococcal cassette chromosome mec element [SCC mec ] remnants) and 98.1% of the MRSA strains ( n = 160, including 10 mecC -positive MRSA) were accurately categorized.

Funder

Bundesministerium für Bildung und Forschung

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02081-15

Reference54 articles.

1. Coagulase-Negative Staphylococci

2. Staphylococcal cassette chromosome mec: Recent advances and new insights

3. A Rapid and High-Throughput Screening Approach for Methicillin-Resistant Staphylococcus aureus Based on the Combination of Two Different Real-Time PCR Assays