Improved Staphylococcus Medium No. 110

Author:

Smuckler Stan A.1,Appleman Milo D.1

Affiliation:

1. Department of Bacteriology, University of Southern California, Los Angeles, California

Abstract

Most media have been unreliable when used to determine the number of coagulase-positive staphylococci in meat pot pies in the presence of overwhelming numbers of Bacillus cells. Various metabolic inhibitors were tested to determine whether they would suppress the growth of Bacillus cells without appreciably affecting the staphylococcal growth. The use of Staphylococcus Medium No. 110 modified by the addition of 0.75 mM sodium azide appears to be practical for the isolation of small numbers of coagulase-positive staphylococci from frozen meat pot pies.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference23 articles.

1. An improved diagnostic and selective medium for isolating coagulase positive staphylococci;BAIRD-PARKER A. C.;J. Appl. Bacteriol.,1962

2. The performance of an egg-yolk-tellurite medium in practical use;BAIRD-PARKER A. C.;J. Appl. Bacteriol.,1962

3. Identification of Staphylococcus pyrogenes by the phosphatase reaction;BARBER M.;J. Pathol. Bacteriol.,1951

4. CARTER C. H. H. V. LEININGER AND B. F. SURKIEWICZ. 1960. The use of staphylococcus medium 110 fortified with egg yolk for isolating coagulase-positive staphylococci. Bacteriol. Proc. p- 45-46.

5. The significance of sodium chloride in studies of staphylococci;CHAPMAN G. H.;J. Bacteriol.,1945

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