Affiliation:
1. The Protein Laboratory, University of Copenhagen, Denmark
2. The Statens Seruminstitut, Department of Clinical Microbiology at Blegdamshospitalet, Copenhagen, Denmark
Abstract
By means of the antigen-antibody crossed electrophoresis procedure of Laurell, 68 antigens were demonstrated in
Candida albicans
. This is about four times the number of antigens described earlier by means of classical immunoelectrophoresis. The procedures for obtaining this result are described, including the preparation of antigen, the immunization of rabbits, and the method of N. M. G. Harboe for the production of purified and concentrated rabbit antibodies suitable for quantitative immunoelectrophoresis. The immunoplates were stained by means of the sensitive Coomassie brilliant blue R. The various quantitative immunoelectrophoretic methods offer considerable possibilities for qualitative and quantitative characterization of antigens, even in complex mixtures, and are therefore well suited for the investigation of microbial antigens.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference12 articles.
1. Les possibilites d'application aux champignons pathogenes de la methode d'Ouchterlony et de l'immunoelectrophorese;Biguet J.;Mykosen,1959
2. etude electrophoritique et immunochimique comparee des antigenes de quelques levures du genre Candida (C. albicans, C. stellatoidea, C. tropicalis, C. zeylanoides, C. krusei, C. pseudotropicalis, C. macedoniensis);Biguet J.;Mycopathologia,1962
3. Etude immunoelectrophoretique de la nature et de l'ordre d'apparition des anticorps precipitants du serum de lapin en fonction de leur mode d'immunisation contre Candida albicans;Biguet J.;Sabouraudia,1965
4. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis;Chrambach A.;Anal. Biochem.,1967
5. Quantitative immunoelectrophoresis of human serum proteins;Clarke H. G. M.;Clin. Sci.,1968
Cited by
42 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献