Mutagenesis and Molecular Modeling Reveal Three Key Extracellular Loops of the Membrane Receptor HasR That Are Involved in Hemophore HasA Binding-Reference-Cited by-同舟云学术

Mutagenesis and Molecular Modeling Reveal Three Key Extracellular Loops of the Membrane Receptor HasR That Are Involved in Hemophore HasA Binding

Published:2007-07-15 Issue:14 Volume:189 Page:5379-5382
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Barjon Clément¹,Wecker Karine²,Izadi-Pruneyre Nadia²,Delepelaire Philippe¹

Affiliation:

1. Unité des Membranes Bactériennes, Département de Microbiologie, CNRS URA2172

2. Unité de Résonance Magnétique Nucléaire des Biomolécules, Département de Biologie Structurale, CNRS URA 2185, Institut Pasteur, 25-28 rue du Dr. Roux, 75724 Paris Cedex 15, France

Abstract

ABSTRACT On the basis of the three-dimensional model of the heme/hemophore TonB-dependent outer membrane receptor HasR, mutants with six-residue deletions in the 11 putative extracellular loops were generated. Although all mutants continued to be active TonB-dependent heme transporters, mutations in three loops abolished hemophore HasA binding both in vivo and in vitro.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00251-07

Reference18 articles.

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