Abstract
Planktonic bacteria from an estuary were concentrated on membrane filters and counted with both a scanning electron microscope and an epi-illuminated fluorescent microscope. Counts on 0.2 micron Nuclepore filters (polycarbonate) were significantly higher (P less than 0.001) than counts on 0.2-micron Sartorius filters (cellulose). In contrast, there was not a statistically significant difference between the two techniques when Nuclepore filters were used (0.5 less than P less than 0.9). The average cell volume from this study area was 0.047 micron3. The estimated number of bacteria ranged from 10(6) to 10(7) bacteria per ml, representing from 4 to 40 mg of C per m3.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference19 articles.
1. Techniques for the preservation of three-dimensional structure in preparing specimens for the electron microscope;Anderson T. F.;Trans. N. Y. Acad. Sci. Ser. II,1951
2. Cohen A. L. 1974. Critical point drying p. 44-112. In M. A. Hayat (ed.) Principles and techniques of scanning electron microscopy: biological applications vol. 1. Van Nostrand Reinhold Co. New York.
3. A rapid critical point method using fluorocarbons ("Freons;Cohen A. L.;J. Microsc. (Paris),1968
4. Direct counts of aquatic bacteria by a modified epi-fluorescent technique;Daley R. J.;Limnol. Oceanogr.,1975
5. Doetsch R. N. and T. M. Cook. 1973. Introduction to bacteria and their ecobiology. University Park Press Baltimore.
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