Author:
Wong Diana D.,van Zuylen Wendy J.,Hamilton Stuart T.,Steingruber Mirjam,Sonntag Eric,Marschall Manfred,Rawlinson William D.
Abstract
ABSTRACT
Mutations in the cytomegalovirus UL97 kinase gene contribute to antiviral resistance. Mutations A594S and G598D from two clinical isolates were analyzed, and bacterial artificial chromosome (BAC)-engineered A594S recombinant cytomegalovirus exhibited a ganciclovir-resistant phenotype on plaque reduction. Viral replication was comparable to that of the wild type. Cell-based kinase activity and autophosphorylation of ectopically expressed proteins showed that mutants retained some kinase activity. This study showed that patient-derived cytomegalovirus with different ganciclovir sensitivities retained replication efficiency and exhibited some kinase activity in vitro.
Funder
Australian Government Research Training Program Scholarship
Australian National Health and Medical Research Council
Australia-Germany Joint Research Cooperation Scheme
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
1 articles.
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