Sensitivity and specificity of pooled versus individual sera in a human immunodeficiency virus antibody prevalence study

Author:

Cahoon-Young B1,Chandler A1,Livermore T1,Gaudino J1,Benjamin R1

Affiliation:

1. Public Health Laboratory, Alameda County Health Care Services Agency, Oakland, California 94607.

Abstract

We evaluated the efficacy of testing pooled versus individual sera for the detection of human immunodeficiency virus antibody. A total of 5,000 individual specimens and 500 pools of 10 specimens each were assayed by an enzyme-linked immunosorbent assay. There was complete agreement in human immunodeficiency virus enzyme-linked immunosorbent assay reactivity for pooled versus individual specimens. An estimated savings of 60 to 80% (labor and supplies) can be realized dependent upon pooling and assay format.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

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3. Comparison of two commercially available anti-HIV ELISAs: Abbott HTLV-111 EIA and DuPont HTLV-III-ELISA;Burkhardt U.;J. Med. Virol.,1987

4. AIDS serology testing in low and high risk groups;Carison J. R.;J. Am. Med. Assoc.,1985

5. Safety of therapeutic immune globulin preparations with respect to transmission of human T-lymphotropic virus type IIl/lymphadenopathy-associated virus infection;Centers for Disease Control.;Morbid. Mortal. Weekly Rep.,1986

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