Production of Axenic Gonyaulax Cultures by Treatment with Antibiotics

Author:

Divan Charles L.1,Schnoes Heinrich K.1

Affiliation:

1. Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706

Abstract

The effects of amphotericin B, chloramphenicol, dihydrostreptomycin sulfate, neomycin sulfate, polymyxin B sulfate, potassium penicillin G, and streptomycin sulfate (used singularly and in various combinations at different concentrations) on the growth and development of four marine dinoflagellates of the genus Gonyaulax and associated bacteria were studied. The combination of amphotericin B, dihydrostreptomycin, neomycin, and penicillin G was highly effective in eliminating bacteria and fungi without reducing dinoflagellate growth and provided a useful method for obtaining axenic cultures of two Gonyaulax species, G. catenella and G. excavata.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference15 articles.

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3. A procedure for routine purification of algae cultures with antibiotics;Droop M. R.;J. Phycol. Bull.,1967

4. Horwitz W. (ed.). 1980. Official methods of analysis of the Association of Official Analytical Chemists p. 851. Association of Official Analytical Chemists Washington D.C.

5. Prakash A. J. C. Medcof and A. D. Tennant. 1971. Paralytic shellfish poisoning in eastern Canada. Fish. Res. Board Can. Bull. p. 177.

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