Affiliation:
1. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Abstract
We investigated the effect of a pH 4-treated human immunoglobulin preparation for intravenous use (pH4-G) on the production of tumor necrosis factor alpha (TNF-alpha) in vivo. The level of TNF-alpha in the sera of rabbits receiving pH4-G before lipopolysaccharide (LPS) injection was lower than that in rabbits receiving only LPS. Similarly, the in vitro production of TNF-alpha was suppressed by pH4-G. Rabbit peritoneal exudate cells stimulated with LPS in the presence of pH4-G produced less TNF-alpha than did those stimulated only with LPS. pH4-G, however, had no effect on various TNF-alpha activities, such as cytotoxicity against tumorigenic murine fibroblasts (L929 cells), induction of interleukin-1 production, or fever induction. These results indicate that pH4-G suppresses TNF-alpha production without affecting TNF-alpha activities. A suppressive effect on the expression of TNF-alpha mRNA was also observed.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference21 articles.
1. Tumor necrosis factor stimulates interleukin 1 and prostaglandin E2 production in resting macrophages;Bachwich P. R.;Biochem. Biophys. Res. Commun.,1986
2. The common mediator of shock, cachexia and tumor necrosis;Beutler C. A.;Adv. Immunol.,1988
3. An endotoxin-induced serum factor that causes necrosis of tumors;Carswell E. A.;Proc. Natl. Acad. Sci. USA,1975
4. Lymphocyte Fcy-receptor blocking antibodies in intravenous gammaglobulin preparations;Cocker J. E.;J. Clin. Lab. Immunol.,1987
5. Cell-associated tumor necrosis factor (TNF) as a killing mechanism of activated cytotoxic macrophages;Decker T.;J. Immunol.,1987
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