Variations in Genetic Recombination Due to Amber Mutations in T4D Bacteriophage

Abstract

Recombination experiments were performed to assess the affect of amber mutations in 12 genes of T4D bacteriophage on genetic recombination. Crosses were performed in various suppressor-containing bacterial hosts to permit the production of progeny phage. Amber mutations in genes 32, 46 , and 47 caused decreased recombination, amber mutations in genes 30, 41, 42, 43, 56, 61 , and 62 caused increased recombination, whereas mutations in genes 63 and 37 showed no demonstrable effect on recombination.