Genome Sequence of the Parainfluenza Virus 5 Strain That Persistently Infects AGS Cells

Author:

Wignall-Fleming Elizabeth12,Young Dan F.2,Goodbourn Steve3,Davison Andrew J.1,Randall Richard E.2

Affiliation:

1. MRC – University of Glasgow Centre for Virus Research, Glasgow, United Kingdom

2. Centre for Biomolecular Sciences, School of Biology, University of St. Andrews, St. Andrews, United Kingdom

3. Division of Basic Medical Sciences, St. George's, University of London, London, United Kingdom

Abstract

ABSTRACT We have sequenced the parainfluenza virus 5 strain that persistently infects the commonly used AGS human cell line without causing cytopathology. This virus is most closely related to human strains, indicating that it may have originated from biopsy material or from laboratory contamination during generation of the cell line.

Funder

Wellcome Trust

Publisher

American Society for Microbiology

Subject

Genetics,Molecular Biology

Reference9 articles.

1. Wang L-F, Collins PL, Fouchier RAM, Kurath G, Lamb RA, Randall RE, Rima BK. 2012. Family Paramyxoviridae, p 672–685. In King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (ed), Virus taxonomy, ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic, London, United Kingdom.

2. Parks GD, Manuse MJ, Johnson JB. 2011. The parainfluenza virus simian virus. In Samal SK (ed), The biology of paramyxoviruses, vol 5. Caister Academic Press, Norfolk, United Kingdom.

3. Relationships and host range of human, canine, simian and porcine isolates of simian virus 5 (parainfluenza virus 5)

4. AGS and other tissue culture cells can unknowingly be persistently infected with PIV5; A virus that blocks interferon signalling by degrading STAT1

5. Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach;Barranco SC;Cancer Res,1983

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