High-Quality Genome Assembly and Annotation for Plasmodium coatneyi, Generated Using Single-Molecule Real-Time PacBio Technology

Author:

Chien Jung-Ting12,Pakala Suman B.32,Geraldo Juliana A.4,Lapp Stacey A.12,Humphrey Jay C.32,Barnwell John W.52,Kissinger Jessica C.32,Galinski Mary R.162

Affiliation:

1. International Center for Malaria Research, Education and Development, Emory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, Georgia, USA

2. Malaria Host–Pathogen Interaction Center, Emory University, Atlanta, Georgia, USA

3. Department of Genetics, Institute of Bioinformatics, Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, USA

4. Biosystems Informatics & Genomics, René Rachou Research Center (CPqRR-FIOCRUZ), Belo Horizonte, Minas Gerais, Brazil

5. Malaria Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA

6. Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA

Abstract

ABSTRACT Plasmodium coatneyi is a protozoan parasite species that causes simian malaria and is an excellent model for studying disease caused by the human malaria parasite, P. falciparum . Here we report the complete (nontelomeric) genome sequence of P. coatneyi Hackeri generated by the application of only Pacific Biosciences RS II (PacBio RS II) single-molecule real-time (SMRT) high-resolution sequence technology and assembly using the Hierarchical Genome Assembly Process (HGAP). This is the first Plasmodium genome sequence reported to use only PacBio technology. This approach has proven to be superior to short-read only approaches for this species.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Genetics,Molecular Biology

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