Affiliation:
1. New England Biolabs, Ipswich, Massachusetts, USA
Abstract
ABSTRACT
SHuffle strains are genetically engineered
Escherichia coli
strains that are capable of oxidizing cysteines within proteins to form disulfide bonds. Here we present the complete genome of both the K-12 and B versions of SHuffle strains along with their parental ancestors. These strains have been of significant use to both the general scientific community and the biotech industry, interested in producing novel disulfide-bonded proteins that were hitherto unable to be expressed in standard
E. coli
expression strains.
Publisher
American Society for Microbiology
Subject
Genetics,Molecular Biology
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献