Use of Integrated Cell Culture-PCR To Evaluate the Effectiveness of Poliovirus Inactivation by Chlorine-Reference-Cited by-同舟云学术

Use of Integrated Cell Culture-PCR To Evaluate the Effectiveness of Poliovirus Inactivation by Chlorine

Published:2000-05 Issue:5 Volume:66 Page:2267-2268
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Blackmer Felisa¹,Reynolds Kelly A.¹,Gerba Charles P.¹,Pepper Ian L.¹

Affiliation:

1. Department of Soil, Water and Environmental Science, The University of Arizona, Tucson, Arizona 85721

Abstract

ABSTRACT Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.5.2267-2268.2000

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5. Cell culture and PCR determination of poliovirus inactivation by disinfectants

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