Bacterial Calcium Transport: Energy-Dependent Calcium Uptake by Membrane Vesicles from Bacillus megaterium

Author:

Golub Ellis E.1,Bronner Felix1

Affiliation:

1. Department of Oral Biology, School of Dental Medicine, The University of Connecticut Health Center, Farmington, Connecticut 06032

Abstract

Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosulfate are needed as a source of electrons for the energy-dependent increase in calcium uptake. Addition of 8 mM sodium cyanide inhibited the energy-dependent uptake. If this calcium uptake mechanism is a component of the sporulation-specific calcium accumulation process, the latter's functional expression would appear to be inhibited during log-phase growth.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference9 articles.

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5. Eisenstadt E. and S. Silver. 1972. Calcium transport during sporulation in Bacillus subtilis p. 180-186. In L. L. Campbell (ed.) Spores V. American Society for Microbiology Washington D.C.

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2. Characterization of a P-type Ca(2+)-ATPase from Flavobacterium odoratum.;Journal of Biological Chemistry;1992-08

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