Tetracycline Susceptibility Testing and Resistance Genes in Isolates of Acinetobacter baumannii - Acinetobacter calcoaceticus Complex from a U.S. Military Hospital

Author:

Akers Kevin S.1,Mende Katrin2,Yun Heather C.13,Hospenthal Duane R.13,Beckius Miriam L.1,Yu Xin1,Murray Clinton K.13

Affiliation:

1. San Antonio Military Medical Center, Fort Sam Houston, Texas

2. Infectious Disease Clinical Research Program, San Antonio, Texas

3. Uniformed Services University of the Health Sciences, Bethesda, Maryland

Abstract

ABSTRACT Infections with multidrug-resistant Acinetobacter baumannii - Acinetobacter calcoaceticus complex bacteria complicate the care of U.S. military personnel and civilians worldwide. One hundred thirty-three isolates from 89 patients at our facility during 2006 and 2007 were tested by disk diffusion, Etest, and broth microdilution for susceptibility to tetracycline, doxycycline, minocycline, and tigecycline. Minocycline was the most active in vitro, with 90% of the isolates tested susceptible. Susceptibilities varied significantly with the testing method. The acquired tetracycline resistance genes tetA, tetB , and tetA (39) were present in the isolates.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference13 articles.

1. Agersø, Y., and A. Petersen. 2007. The tetracycline resistance determinant Tet 39 and the sulphonamide resistance gene sulII are common among resistant Acinetobacter spp. isolated from integrated fish farms in Thailand. J. Antimicrob. Chemother.59:23-27.

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3. Performance standards for antimicrobial disk susceptibility tests: approved standard (M2-A9) 2006

4. Performance standards for antimicrobial susceptibility testing: seventeenth informational supplement (M100-S17). 2007

5. Griffith, M. E., H. C. Yun, L. L. Horvath, and C. K. Murray. 2008. Minocyline therapy for traumatic wound infections caused by the multidrug-resistant Acinetobacter baumannii-Acinetobacter calcoaceticus complex. Infect. Dis. Clin. Pract.16:16-19.

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