Binding of Homologous Polymerized Deoxyribonucleic Acid by Streptomyces griseus

Abstract

Germaine , G. R. (University of Minnesota, Minneapolis), and D. L. Anderson . Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus . J. Bacteriol. 92: 662–667. 1966—An irreversible P 32 deoxyribonucleic acid (DNA) binding system is described for Streptomyces griseus S104. The 69% guanine plus cytosine (GC) S. griseus DNA was bound by late exponential –early stationary phase cultures. Simultaneous addition of deoxyribonuclease with the P 32 -DNA to maximally receptive cultures reduced the uptake by 98%. Saturation of the binding ability occurred after 20-min incubation of cells with P 32 -DNA. Preparative cesium chloride density gradient centrifugation of the P 32 -DNA revealed that 98% of the label banded in the position expected for S. griseus DNA. Sedimentation of the P 32 -DNA at both 23 and 37 μg/ml indicated a molecular weight of 10.8 million. Attempts to transform S. griseus S104 auxotrophs to prototrophy by use of immediate and delayed selective procedures were unsuccessful.