Author:
Chejanovsky N,Beigel M,Loyter A
Abstract
Sendai virus particles bind to human erythrocytes at 4 degrees C and fuse with them at 37 degrees C. The present work describes a new method by which adsorbed virus particles can be removed from human erythrocytes, allowing quantitative determination of the number of virus particles which can bind and fuse with human erythrocyte membranes. Through the use of 125I-labeled Sendai virus particles, it is shown that incubation with 50 mM dithiothreitol removed about 90 to 95% of adsorbed virus particles. Fused virus particles were resistant to treatment with dithiothreitol. Negligible amounts of 125I-labeled Sendai virus particles were removed by treatment with dithiothreitol after incubation of virus-cell complexes at 37 degrees C. Trypsinized virus particles were able to attach to, but not fuse with, human erythrocytes even after prolonged incubation at 37 degrees C. Treatment with dithiothreitol removed as much as 80 to 85% of trypsinized virus particles incubated with human erythrocytes at 37 degrees C. A quantitative determination revealed that about 1,000 to 1,200 and 600 to 800 Sendai virus particles can bind to or fuse with human erythrocytes, respectively.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference16 articles.
1. Ginsberg D. D. J. Volsky N. Zakai Y. Laster and A. Loyter. 1979. The association between enveloped viruses and host cell membranes: a specific ligand receptor interaction p. 32-42. In G. Pepeu M. J. Kuhar and S. J. Enna (ed.) Receptors neurotransmitters and peptide hormones. Raven Press New York.
2. On the entry of Semliki Forest virus into BHK-21 cells;Helenius A.;J. Cell Biol.,1980
3. Influenza viruses cause hemolysis and fusion of cells;Huang R. T. C.;Virology,1981
4. The use of fusion methods for microinjection of macromolecules into animal cells;Kulka R. G.;Curr. Top. Membr. Transp.,1979
5. Restoration of the fusion capacity of human erythrocyte ghosts by the SH-blocking reagents;Lalazar A.;Exp. Cell Res.,1977
Cited by
20 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献