Evaluation of 16S rRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species-Reference-Cited by-同舟云学术

Evaluation of 16S rRNA Gene-Based PCR Assays for Genus-Level Identification of Helicobacter Species

Published:2008-05 Issue:5 Volume:46 Page:1867-1869
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Moyaert H.¹,Pasmans F.¹,Ducatelle R.¹,Haesebrouck F.¹,Baele M.¹

Affiliation:

1. Department of Pathology, Bacteriology, and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium

Abstract

ABSTRACT The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10 3 and 1 viable bacterial cell per reaction mixture.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00139-08

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