Affiliation:
1. College of Plant Sciences, Jilin University, Changchun, China
Abstract
ABSTRACT
Aquaglyceroporins (GlpFs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. Fungal species form a large group of eukaryotic organisms, and their GlpFs may be diverse, exhibiting various activities. However, few filamentous fungal GlpFs have been biologically investigated. Here, a
glpF
gene from the halophilic fungus
Aspergillus glaucus
(
AgglpF
) was verified to be a channel of water or glycerol in
Xenopus laevis
oocytes and was further functionally analyzed in three heterologous systems. In
Saccharomyces cerevisiae
, cells overexpressing
AgglpF
possessed significant tolerance of drought, salt, and certain metal ions.
AgglpF
was then characterized in the filamentous fungus of
Neurospora crassa
. Based on the
N. crassa
aquaporin gene (
NcAQP
) disruption mutant (the Δ
aqp
mutant), a series of complementary strains carrying
NcAQP
and
AgglpF
and three asparagine-proline-alanine-gene (
NPA
)-deleted
AgglpF
fragments were created. As revealed by salt resistance analysis, the
AgglpF
complementary strain possessed the highest salt resistance among the tested strains. In addition, the intracellular glycerol content in the
AgglpF
complementary strain was markedly higher than that in the other strains. The AgGlpF-green fluorescent protein (GFP) fusion protein was subcellularly localized in the plasma membrane of onion epidermal cells, suggesting that
AgglpF
functions in plants. Indeed, when
AgglpF
was expressed in
Arabidopsis thaliana
, transgenic lines survived under conditions of high osmotic stress and under conditions of drought stress in particular. Overall, our results revealed that AgGlpF as a water/glycerol transporter is required for survival of both fungi and plants under conditions of high osmotic stress and may have value in applications in genetic engineering for generating high salt and drought resistance.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
16 articles.
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