Affiliation:
1. Microbiology Laboratory Hospital Severo Ochoa (Leganés), Madrid
2. Department of Internal Medicine
3. Microbiology Laboratory, Hospital Virgen de la Luz, Cuenca, Spain
Abstract
ABSTRACT
The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated Rose Bengal test, microagglutination test, microtiter-adapted Coombs test, and immunocapture-agglutination test (Brucellacapt) were positive for all sera from patients with acute brucellosis. The immunoglobulin G (IgG), IgM, and IgA commercial enzyme immunoassays (ELISAs) failed to show specific antibodies in 3 patients, 10 patients, and 1 patient, respectively. The sensitivity of ELISA is not higher than that of conventional tests.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
Reference20 articles.
1. Al Skait, M. A. 1999. Seroepidemiological survey of brucellosis antibodies in Saudi Arabia. Ann. Saudi Med.19:219-222.
2. Araj, G. F., A. R. Lulu, M. Y. Mustafa, and M. I. Khateeb. 1986. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. J. Hyg.97:457-469.
3. Bettelheim, K. A., W. J. Maskill, and J. Pearce. 1983. Comparison of standard tube and microagglutination techniques for determining Brucella antibodies. J. Hyg.90:33-39.
4. Cooper, C. W. 1992. Prevalence of antibody to Brucella in asymptomatic well individuals in Saudi Arabia. J. Trop. Med. Hyg.95:140-142.
5. Díaz, R., and I. Moriyon. 1989. Laboratory techniques in the diagnosis of human brucellosis, p. 73-83. In E. J. Young and M. J. Corbel (ed.), Brucellosis: clinical and laboratory aspects. CRC Press, Boca Raton, FL.
Cited by
97 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献