Micro Indirect Hemagglutination Test for Cytomegalovirus-Reference-Cited by-同舟云学术

Micro Indirect Hemagglutination Test for Cytomegalovirus

Published:1971-01 Issue:1 Volume:21 Page:104-107
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Fuccillo David A.¹²,Moder Flora L.¹²,Traub Renee G.¹²,Hensen Sally¹²,Sever John L.¹²

Affiliation:

1. Section on Infectious Diseases, Perinatal Research Branch, National Institute of Neurological Diseases and Stroke, Bethesda, Maryland 20014

2. Microbiological Associates, Incorporated, Bethesda, Maryland 20014

Abstract

In an effort to obtain the flexibility and ease of performance of a rapid, serological test for detection of cytomegalovirus antibody, the indirect hemagglutination (IHA) technique was investigated by using a microserological system. Antigens were prepared from tissue cultures of infected human fibroblasts. The specificity of the cytomegalovirus antibody response detected by the IHA test correlated well with the standard neutralization test. The IHA method was more sensitive than the complement fixation test in detecting antibody in congenitally infected newborns. There appeared to be some heterologous antibody response with Herpesvirus hominis or varicella virus infections. The IHA test pattern was found to be very stable with excellent persistence of agglutination.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.21.1.104-107.1971

Reference9 articles.

1. Birnbaumn G. J. I. Lynch A. M. Margileth W. M. Lonergan and J. L. Sever. 1969. Cytomegalovirus infections in newborn infants. J. Pediat. 75:789-795.

2. Minicultures of mammalian cells in a new plastic plate;Fuccillo D. A.;Appl. Microbiol.,1969

3. Herpesvirus hominis type I and II: a specific microindirect hemagglutination test;Fuccillo D. A.;Proc. Soc. Exp. Biol. Med.,1970

4. Cytomegaloviremia following congenital infection;Lang D. J.;J. Pediat.,1968

5. Schmidt N. J. 1964. Tissue culture methods and procedures for diagnostic virology p. 78-176. In E. H. Lennette and N. J. Schmidt (ed.) Diagnostic procedures for viral and rickettsial diseases 3rd ed. American Public Health Association Inc. New York.

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