Affiliation:
1. Department of Medicine, Harvard Medical School, and Medical Service (Infectious Disease Unit), Massachusetts General Hospital, Boston, Massachusetts 02114
Abstract
Actively growing aerobic and anaerobic bacteria were screened by a plate assay, with reconstituted guinea pig collagen as a substrate, for their ability to produce a collagenolytic factor. Collagenolytic activity was not demonstrated among the aerobic organisms tested, with the exception of one strain of
Staphylococcus aureus
(only when grown under anaerobic conditions). Collagenolytic activity, however, was detected in cultures of
Clostridium tetani
and
Bacteroides
species other than
B. melaninogenicus
. Collagenolytic activity of these organisms could be confirmed by measuring the amount of hydroxyproline liberated from the collagen gel during growth. Although collagenase production by
Pseudomonas aeruginosa
has been suggested in previous reports, our results were negative. An extracellular fraction of
P. aeruginosa
was able to hydrolyze a synthetic hexapeptide Cbz-glycyl-
l
-prolyl-glycyl-glycyl-
l
-prolyl-
l
- alanine, but was without detectable effect on reconstituted collagen.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
31 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献