Occurrence of Phosphonosphingolipids in Bdellovibrio bacteriovorus Strain UKi2

Author:

Steiner S.1,Conti S. F.1,Lester Robert L.1

Affiliation:

1. School of Biological Sciences and Department of Biochemistry, College of Medicine, University of Kentucky Lexington, Kentucky 40506

Abstract

The major phospholipids of two strains of Bdellovibrio bacteriovorus were characterized. Both strain UKi1, which is obligately saprophytic, and strain UKi2, which is facultatively parasitic, contained phosphatidylethanolamine and phosphatidylglycerol as their major glycerophosphatides. A branched, 15-carbon fatty acid is the major component of these alkali-labile lipids. Absent from UKi1 but present in UKi2 were three alkali-stable lipids (compounds 8, 9, and 11) which appear to be phosphosphingolipids. After acid hydrolysis, both compound 8 and 9 yield the identical phosphorus-containing substance that is water soluble, dipolar ionic, and ninhydrin positive. This substance appears to contain a C-P bond since P i could not be released from this substance by treatment with alkaline phosphatase or by very harsh mineral acid treatment. Based on chromatographic comparisons, this phosphonate appears to be a novel lipid constituent. Upon degradation, compound 8 yields 1 mol of dihydroxy long-chain base and compound 9 yields 1 mol of a trihydroxy long-chain base. These bases appear to have a 17-carbon, possibly branched, structure based on gas-liquid chromatography retention times. Degradation of both sphingolipids yields a mixture of hydroxy fatty acids, the major component being a branched, 15-carbon hydroxy acid.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference31 articles.

1. Phosphorous assay in column chromatography;Bartlett G. R.;J. Biol. Chem.,1959

2. Ultrastructure and cell division of a facultatively parasi+.c strain of Bdellovibrio bacteriovorus;Bumham J. C.;J. Bacteriol.,1970

3. Improved reagent for trimethylsilylation of sphingolipid bases;Carter H. E.;J. Lipid Res.,1967

4. Facultatively parasitic strain of Bdellovibrio bacteriovorus;Diedrich D. L.;J. Bacteriol.,1969

5. Colorimetric method for determination of sugars and related substances;Dubois M.;Anal. Chem.,1956

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