Comparison of Two Multilocus Sequence Typing Schemes for Mycoplasma bovis and Revision of the PubMLST Reference Method-Reference-Cited by-同舟云学术

Comparison of Two Multilocus Sequence Typing Schemes for Mycoplasma bovis and Revision of the PubMLST Reference Method

Published:2020-05-26 Issue:6 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Register Karen B.¹,Lysnyansky Inna²,Jelinski Murray D.³,Boatwright William D.¹,Waldner Matthew³,Bayles Darrell O.⁴,Pilo Paola⁵,Alt David P.⁴

Affiliation:

1. Ruminant Diseases and Immunology Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, Ames, Iowa, USA

2. Department of Avian Diseases, Kimron Veterinary Institute, Beit Dagan, Israel

3. Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

4. Infectious Bacterial Diseases Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, Ames, Iowa, USA

5. Department of Infectious Diseases and Pathobiology, Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland

Abstract

Mycoplasma bovis causes pneumonia, pharyngitis, otitis, arthritis, mastitis, and reproductive disorders in cattle and bison. Two multilocus sequence typing (MLST) schemes have been developed for M. bovis , with one serving as the PubMLST reference method, but no comparison of the schemes has been undertaken. Although the PubMLST scheme has proven to be highly discriminatory and informative, the recent discovery of isolates missing one of the typing loci, adh-1 , raises concern about its suitability for continued use.

Funder

United States Department of Agriculture

Israeli Ministry of Agriculture and Rural Development

Saskatchewan Ministry of Agrculture

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00283-20

Reference38 articles.

1. Mycoplasma bovis Infections in Cattle