Molecular Characterization of Isolates of Waterborne Cryptosporidium spp. Collected during an Outbreak of Gastroenteritis in South Burgundy, France

Author:

Dalle Frédéric1,Roz Pascale1,Dautin Guillaume1,Di-Palma Marc2,Kohli Evelyne3,Sire-Bidault C.4,Fleischmann Marie George5,Gallay Anne6,Carbonel Sylvia2,Bon Fabienne3,Tillier Claude5,Beaudeau Pascal6,Bonnin Alain1

Affiliation:

1. Laboratoire de Parasitologie Mycologie et Laboratoire de Microbiologie Médicale et Moléculaire (EA 562), CHU et Faculté de Médecine

2. DRASS de Bourgogne, CIR Centre Est

3. Laboratoire de Virologie et Laboratoire de Microbiologie Médicale et Moléculaire (EA 562), CHU et Faculté de Pharmacie, Dijon

4. Laboratoire de Biologie, Centre Hospitalier de Chalon sur Saône, Chalon sur Saône

5. DDASS de Saône et Loire, Macon

6. Institut de Veille Sanitaire, Saint Maurice, France

Abstract

ABSTRACT In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs targeting the Hsp70 and the 18S rRNA genes of Cryptosporidium . Sequencing of the PCR fragments produced sequences identical to that of Cryptosporidium parvum genotype 1.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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