Affiliation:
1. Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South Africa
2. MP International Consultancy, 6 Normandale House, Normandale, Bexhill-on-Sea, East Sussex TN39 3NZ, United Kingdom
Abstract
ABSTRACTIn a previous paper, we reported on a large number of cheetah blood specimens that gave positive signals only forBabesiaand/orTheileriagenus-specific probes on the reverse line blot (RLB) assay, indicating the presence of a novel species or variant of an existing species. Some of these specimens were investigated further by microscopic, serological, sequencing, and phylogenetic analyses. The near-full-length 18S rRNA genes of 13 samples, as well as the second internal transcribed spacer (ITS2) region, were amplified, cloned, and sequenced. A species-specific RLB probe, designed to target the hypervariable V4 region of the 18S rRNA gene for detection of the novelBabesiasp., was used to screen an additional 137 cheetah blood specimens for the presence of the species. The prevalence of infection was 28.5%. Here we describe the morphology and phylogenetic relationships of the novel species, which we have namedBabesia lengausp. nov.
Publisher
American Society for Microbiology
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