Affiliation:
1. Departments of Laboratory Medicine
2. Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea
3. Internal Medicine, Chonnam National University Medical School, Gwangju, Korea
Abstract
ABSTRACT
Data on clinical isolates of
Kodamaea
(
Pichia
)
ohmeri
, an emerging fungal pathogen, are scarce. Over the past 5 years, we identified yeast isolates from nine patients with fungemia as
K. ohmeri
by using the API 20C system. Here, we reanalyzed these isolates first by sequencing the internal transcribed spacer 2 (ITS2) regions and then by growing the isolates on CHROMagar
Candida
medium and subjecting them to pulsed-field gel electrophoresis (PFGE). Based on their ITS2 sequences, six of the nine isolates were confirmed as
K. ohmeri
, while the others were identified as
Candida haemulonii
(
n
= 2) and
Candida parapsilosis
(
n
= 1). PFGE karyotyping of the
K. ohmeri
isolates revealed similar major bands, and their colonies showed a characteristic color change from pink to blue when grown on CHROMagar
Candida
medium for more than 48 h. For
K. ohmeri
, the ranges of MICs of fluconazole, voriconazole, caspofungin, and micafungin were 2 to 32 μg/ml, 0.03 to 0.5 μg/ml, 0.125 to 0.25 μg/ml, and 0.03 to 0.06 μg/ml, respectively. Restriction endonuclease analysis of genomic NotI-digested DNA (REAG-N) from isolates from different patients produced unique patterns, suggesting that the fungemia had occurred sporadically. This study determined that ITS2 sequence data, PFGE karyotypes, and CHROMagar
Candida
chromogenic culture medium are reliable diagnostic tools for identifying
K. ohmeri
while REAG-N is useful for genotyping the clinical isolates of
K. ohmeri
.
Publisher
American Society for Microbiology
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