Author:
Guillet J G,Hoebeke J,Tram C,Marullo S,Strosberg A D
Abstract
Hybridoma-producing monoclonal antibodies against Legionella pneumophila were produced by the fusion of nonsecreting mouse myeloma cells (NS-1) with splenocytes of BALB/c mice immunized by heat-killed L. pneumophila of serogroup I. Of 96 wells, 85 produced clones, of which 28 were positive as measured by an enzyme-linked immunosorbent assay technique. Ten of the hybridoma supernatants, remaining positive after 2 months of culture, were tested against the other Legionella serogroups and the atypical strains. None showed significant cross-reaction. Six of the positive clones were subcloned by limiting dilution, and two subclones were put into ascites in BALB/c mice. The monoclonal antibody obtained from the II-6-18 subclone was of the gamma-3 isotype. In this report, we describe the conditions for the use of this monoclonal antibody as a diagnostic tool for the detection of serogroup I L. pneumophila.
Publisher
American Society for Microbiology
Reference14 articles.
1. Classification of the Legionnaire's disease bacterium: an interim report;Brenner D. J.;Curr. Microbiol.,1978
2. Immunological diagnosis of Legionnaire's disease: cross reactions with anaerobic and micro-aerobic organisms and infections caused by them;Edelstein P. H.;J. Infect. Dis.,1980
3. Epidemiology of Legionnaire's disease;Eickhoff T. C.;Ann. Intern. Med.,1979
4. Scottish experience with the serological diagnosis of Legionnaire's disease;Fallon R. J.;Ann. Intern. Med.,1979
5. Detection of antibodies to Legionnaire's disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests;Farshy C. E.;J. Clin. Microbiol.,1978
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